FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10433882
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8243207.900415205002319No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150160.14391575446224136No Hit
GGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141390.13551044568071596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCTA5800.027.15271820
CCGTATC51000.024.22306655
CGTATCA53250.023.85665556
GGTGCCT6550.023.50932319
CGCCGTA56400.023.20756553
TCGCCGT55600.023.16381852
GCCGTAT60900.022.46955554
GTATCAT53700.021.96231557
CTCGGTG60050.021.68069844
TCATTAA64850.021.6401960
GATCTCG59300.021.54146641
TCTCGGT59400.021.44628143
AGATCTC60950.021.13046640
CGAGATC10750.020.512335
CCGTGTC3250.019.38267755
TATCATT64650.019.21722458
TAGATCT71500.019.13840339
CTAGATC9750.019.026295
TGGTGCC8300.018.97418818
TGTCGGC8500.018.11566230
Produced by FastQC (version 0.11.5)