FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7269739
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190970.2626916867304315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG4400.044.54897726
ACACTCT64750.042.3416561
GCCCACA50700.037.8458671
CCGGGCT5350.035.98415425
GCTTGAA504600.033.03535566
TGCTTGA496650.032.31686865
CTGCTTG505350.032.21759464
GAAAAAA613700.031.59822770
CTTGAAA537600.031.37794167
TGAAAAA600850.031.0916169
TTGAAAA551350.030.90644668
TCTGCTT540200.030.48250663
GCGTGAA44950.029.74262666
AGGCCGC7000.029.49746516
CTGCGTG39450.028.8324864
TGCGTGA35100.028.71646765
TTCTGCT563150.028.68714762
CTTCTGC599250.028.59387661
GTCTTCT610550.028.35661359
CCGTCTT677650.027.5575857
Produced by FastQC (version 0.11.5)