FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7948769
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131250.16511990724601508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT14500.042.7244525
CGGGCTG15050.040.9305326
AGGCCGC16350.037.03204316
GAGGCCG16600.036.89615
CGCCGGG16800.036.45722223
CCGCCGG18050.033.9324822
TCGAGGC19000.032.97226713
CCTACGA17350.032.277536
TCCTACG18150.032.204535
GGGCTGC19200.031.90127427
GCCGGGC20350.030.44152624
GGCCGCC19850.030.32614917
ATCGAGG21250.029.64578212
GCGAGGG7100.028.09738316
GCCCACA38950.027.0577351
GGCTGCA23250.026.34427628
TACGACG21750.024.46117838
CTACGAC23100.024.39534437
ACACTCT42800.023.5603181
TATCGAG27800.023.1644711
Produced by FastQC (version 0.11.5)