FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5767407
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151440.26257900647552707No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA45650.032.060981
GTCGGGG7250.031.37822762
CCGGGCT5900.029.66127825
GGGGTCG7500.029.39898359
TCGAGGC6950.028.2002213
TGGCGGG38650.028.1620355
GGGTGGA22000.028.15811766
CGGGCTG6250.028.00024826
GGGTCGG7850.027.64235760
GGTCGGG6250.027.43905361
CCTACGA5900.027.28884936
GCGTCGG8000.026.68657760
AGGCCGC6900.026.3756716
GGGTGCA9300.026.34317666
TGCCGGG20350.025.79763455
GCGTGCA7600.025.78858466
CCGGGGT16050.025.51291557
CCGGCGT16800.025.20724357
GCGGGGT43500.025.02190857
CGGGGTC16400.024.96843358
Produced by FastQC (version 0.11.5)