FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8665590
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169330.19540504454976523No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT60200.034.4969941
CTGCTTG106700.025.15841564
GCGTGAA30900.024.91819266
GCTTGAA114900.024.67273766
GCCCACA37050.023.1579931
AGTGGCG12100.022.85265439
TGCTTGA112050.022.64531965
GGCGTGA12850.022.06145965
CGGCGTG17150.021.83594364
CACTCTT131150.021.3087351
CGTGAAA38550.021.06266867
GCCGTCT168050.020.93015156
CCGTCTT181850.019.9576957
TCGGCGT19400.019.84464363
CTGCGTG28400.019.71759664
TGCGTGA24950.019.63856765
GAAAAAA245300.019.0756270
CCGGGCT6250.019.0405225
GGCGGTA13800.019.02225942
TGCCGTC217650.018.99059355
Produced by FastQC (version 0.11.5)