FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307434
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97260.22579568253396337No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG3050.028.68985726
GCGTGAA12200.026.67908966
ACACTCT26000.026.1281241
GCCCACA29850.023.9312671
CCGGGCT3750.023.33441725
GCGTGCA6150.023.3322566
GGGCGTG5450.023.11819364
GGGTGGA10800.023.0081966
CGGGGTG17850.022.5474558
CTGCGTG10550.021.8947264
CGTGAAA16750.021.5208967
CCGGGGT10300.021.40627557
GGGCTGC4100.021.34245527
GCGGGGT14600.021.09442157
TGCCGGG10800.021.06334755
TGCGTGA9650.021.03529465
GTCGGCG6500.020.99902562
GCGTGGA7050.020.85009666
GGCGTGA5750.020.6946965
CGTCGGC6000.020.41571861
Produced by FastQC (version 0.11.5)