FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5713893
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127200.22261529923644002No Hit
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG78050.13659688762110175Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA62750.10982004738275639Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT102050.051.0599141
CGGGCTG7950.042.26505326
CCGGGCT8100.041.91447425
AGGCCGC8300.041.74458716
GGGCTGC9150.036.7220927
GAGGCCG10100.036.03753315
GGCCGCC9950.034.4703817
TCGAGGC10750.033.85851713
CGCCGGG10550.032.8422923
GCTTGAA209850.031.03732366
GCCGGGC11750.030.68016224
GAAAAAA304150.030.37800270
TGCTTGA203150.029.82133965
CTGCTTG216200.029.3487164
GCGTGAA41300.029.15112166
TGAAAAA292300.029.02330669
CCGCCGG11900.028.82237622
ATCGAGG12750.028.82187512
CACTCTT229900.028.6662451
CTTGAAA233600.028.4808167
Produced by FastQC (version 0.11.5)