FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4075461
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83020.20370701621239903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT26400.037.801871
GCCCACA20950.035.7686121
GGGTGGA10000.029.0490566
GCGTGAA10850.028.06359566
GCCACAC16400.027.3299261
CGGGCTG4650.024.83911526
CTGCGTG9900.024.03961864
GCGTGCA6000.023.91588466
GGCGTGA4400.023.86285465
GTCTGGG4850.023.8136562
CCGGGCT4900.023.57181425
TCGAGGC5350.022.89560313
CGTGAAA14650.022.69494667
GGGCTGC5050.022.17858127
GGGTGCA4600.022.06449566
GAGGCCG5600.021.8734815
CCTACGA3850.021.8193436
TACGCGC2259.094947E-1221.77706322
CACTCTT63350.021.6676481
CTGGGTG6150.021.62530764
Produced by FastQC (version 0.11.5)