FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485801
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6680.1375048631023814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT850.049.41626725
ACACTCT4250.043.6825141
GAGCGAG855.638867E-1141.17174514
CGGGCTG1050.040.00364726
CGCCGGG1100.038.17743723
GCGAGGG951.8735591E-1036.8378816
CCTACGA1051.4551915E-1136.67378636
AGCGAGG1051.4551915E-1136.66246415
GGGACCC400.002416981635.00319328
GCCGGGC1201.8189894E-1234.99958824
GGCCGCC1201.8189894E-1234.99598717
CCGCCGG1201.8189894E-1234.99598722
GCCCACA3000.033.8608171
GGCTGCA1253.6379788E-1233.60306528
AGGCCGC1305.456968E-1232.30398616
GGGCTGC1206.548362E-1132.08625827
GAGACCC450.004301947531.117156
CGAGGGT1052.0238076E-829.9965617
GGGTGAC1103.1810487E-828.63307820
CTACGAC1352.582965E-1028.52405437
Produced by FastQC (version 0.11.5)