FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993564
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41000.2056618197359102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG4350.049.8840826
CCGGGCT4600.047.93383825
ACACTCT20600.047.426961
GGGCTGC4700.046.169327
AGGCCGC4700.045.42235616
GCGAGGG3100.045.15813416
CGCCGGG4850.044.74138623
TCGAGGC5100.044.6047313
GGCCGCC4800.044.4760617
GCCGGGC5450.040.4579224
CGAGGGT3500.039.99720817
GAGCGAG3550.038.44801714
ATCGAGG6050.038.1791512
CCGCCGG5700.038.06942722
AGCGAGG3600.037.9140215
CCTACGA4850.037.5278636
GGCTGCA6050.035.8670628
GAGGCCG6150.035.85115415
GAGGGTG4300.033.36976218
GGGTGAC4150.032.8909220
Produced by FastQC (version 0.11.5)