FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5798108
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG120460.20775742707793646Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC119930.2068433357916065Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119500.20610171455930107No Hit
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA99220.1711247876031285Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT91820.158362003605314Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT86880.14984198293650275Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC76930.13268121256106302Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA74940.12924905848597507TruSeq Adapter, Index 19 (100% over 39bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC69830.12043583872532213Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA66830.11526173710458654Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT152750.055.985621
CGGGCTG12750.055.17653326
AGGCCGC13750.053.70683316
CCGGGCT13450.052.3048925
GGGCTGC13750.051.16369227
GAGGCCG14750.051.01480515
GGCCGCC14300.050.9069517
CGCCGGG15100.046.8196323
TCGAGGC16450.046.16827413
CCGCCGG15800.045.18836622
ATCGAGG18000.042.38710812
CGCCGCC17050.042.2859820
ACGAGTG8700.042.24215338
CCGCCGC17450.041.5172419
GGCTGCA17300.041.06976328
TCCTACG14250.039.78986435
CGAGTGA9450.038.88960339
CCTACGA13300.038.42142536
TATCGAG20200.038.2904711
CACGAGT9600.038.28195637
Produced by FastQC (version 0.11.5)