FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5809029
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110120.1895669654945775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTG10850.042.5834338
CGAGTGA11050.042.1294539
CGGAGTC10950.041.550227
GAGTCAC10850.041.2880489
ACACTCT35000.040.922051
CACGAGT11100.040.67798637
ACGGAGT11600.039.230766
GTGAGGT11700.039.18924742
GGAGTCA12450.036.5441748
AACGGAG12500.036.4061435
CTGCGTG18800.035.1848466
GCGTGAA22950.034.46433368
CCACGAG12900.034.45902636
GTCACTC12900.034.45546711
CGTATGT13150.034.3327158
ACTCAGC13250.034.07359714
CAAACGG13550.033.3266563
ACGTATG14350.032.19334857
TGCGTGA18350.031.66038167
GCACTTA14400.031.35243868
Produced by FastQC (version 0.11.5)