FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4293081
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97520.22715620786097446No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG3600.047.64071326
ACACTCT39100.039.8464241
CCGGGCT4350.039.42679625
AGGCCGC4700.036.4873616
GCCCACA20200.034.491161
CGCCGGG5100.034.31344223
GGGCTGC5450.031.46946327
GAGGCCG5500.031.18010715
GGCCGCC5750.031.04177517
GCTTGAA81550.028.88253468
CTGCTTG78900.028.6999866
GCGTGAA16250.028.6445868
TGCTTGA78550.028.20341967
CTGCGTG14550.027.42181466
CCGCCGG6550.027.25168222
GCCGGGC6900.025.8696524
TGCGTGA13000.025.03708867
TCGAGGC7300.023.97128713
CGTGAAA20400.023.16049469
TCTGCTT101050.022.78997865
Produced by FastQC (version 0.11.5)