FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4176021
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96210.2303867724803108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT38450.042.7087971
CCGGGCT5450.037.2486925
AGGCCGC5900.034.99757416
TCGAGGC6400.033.35746413
TCCTACG5450.032.7543435
GAGGCCG6550.032.5935515
CGGGCTG6450.031.47370126
GGGTGGA15150.030.0312868
CCTACGA5700.026.40515736
CGCCGGG7700.026.36244223
GGGTGTA11200.026.24849568
GCCCACA22250.025.8080181
GGGCTGC8050.025.65285527
AGTGGCG4650.024.84115439
CCGCCGG8650.024.68096222
GCGGGGT34600.024.07376559
CACTCTT96500.024.0199171
TTGCGGG30100.023.37075457
ATCGAGG9150.023.33199512
GGCCGCC8850.022.54081217
Produced by FastQC (version 0.11.5)