FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5861899
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89200.15216911789165935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA17350.020.7877661
AGTGGCG7400.019.86664839
GCGTGAA13450.019.77586266
CGGGCTG3600.019.44519626
ACACTCT25350.019.062121
CCGGCGT9700.018.7616957
CCGGGCT3650.018.21988325
GGGTGGA8400.017.91567666
GCCACAC18950.017.3695561
GCCGGCG7650.016.92702756
TGCGTGA12350.016.43633865
GCCGTCT27550.016.3873456
CTGCGTG13300.016.31488864
GCGGTAG9100.016.15502243
TGCCGGC13900.016.1140855
TATGCCG44150.015.8540353
GAGATCG42050.015.6505116
AGGGGGG5600.015.6322711
GCGTGCA6050.015.61897265
TGCCGTC39700.015.60353955
Produced by FastQC (version 0.11.5)