FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4811980
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82870.17221601087286315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT2900.036.2073725
ACACTCT25100.033.6241871
GCGTGAA20500.030.90059566
CGGGCTG3450.030.43517926
CTGCTTG83700.029.10220364
GCTTGAA89200.028.79872966
GCCCACA17050.028.7549171
GGCGTGA6250.028.55829665
TGCTTGA83250.027.49385565
TCGGCGT10700.027.47499563
AGGCCGC3950.027.46671716
CGGCGTG10200.027.4493464
GGGCTGC3850.026.36398127
GCGTGCA7500.026.13177366
CTGCGTG19350.026.04495864
TGCGTGA16650.025.85431365
GCCGTCT122700.025.6708456
CGTGAAA26200.025.24658667
CCGTCTT130400.025.04068457
TCTGCTT100700.024.9190763
Produced by FastQC (version 0.11.5)