FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_vk6-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_vk6-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184383
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66380.15863748610010125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG9700.053.04162626
CCGGGCT10250.050.1954925
AGGCCGC10650.048.96457316
CGCCGGG10650.048.6365323
GAGGCCG11700.045.46769715
GGCCGCC11950.043.3450117
GCCGGGC12000.043.1649224
CCTACGA11400.042.6762636
GGGCTGC12200.042.45932827
CCGCCGG12600.041.38721522
TCGAGGC13250.040.9412513
TCCTACG13300.037.8954735
GCGAGGG6400.036.63870616
CGAGGCC14450.036.57247514
GGCTGCA14300.036.2240428
ATCGAGG15200.035.22841612
TACGACG13450.033.83005538
CGAGGGT7750.032.51442717
CGCCGCC15950.032.25536320
CTACGAC14650.032.25356737
Produced by FastQC (version 0.11.5)