FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10433882
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105821710.142121599611727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT5800.031.42045225
CACTCTT76750.025.6408461
CCTACGA5250.025.38290636
ACACTCT56600.024.2704891
GCCCACA22550.024.1813411
TCCTACG6250.023.5631935
AGGCCGC9300.022.15552716
CGGGCTG8100.022.06762726
CCCATGA10900.021.806441
GGGCTGC8500.021.02945727
GCCACAC21900.020.5896021
CTACGAC6900.019.82704537
CGAGGCC11450.018.6051514
GGCTGCA9900.018.41130628
AAAGATC33150.018.3370115
TGCATCC9650.018.16163631
GTCACGA41900.018.07826636
CCGAGAC4500.017.91604829
GGAGATC14050.017.902725
GCATCCT10700.017.03574432
Produced by FastQC (version 0.11.5)