FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6221982
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105750.1699619188869399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC40900.017.3277268
TCGGAAG55850.013.2027243
ACGTCTG25350.012.77344114-15
GATCGGA54800.012.7683311
GAACTCC26150.012.73642120-21
GAGCACA61550.012.668569
CGGAAGA61600.012.2005264
CCAGTCA22550.012.04671926-27
ATCGGAA64150.011.8632952
CACGTCT28300.011.77600614-15
GCACACG29950.011.67975410-11
ACACGTC29750.011.44181612-13
CGTCTGA27950.011.41264616-17
CACACGT31700.011.18539812-13
CTCCAGT30200.010.94951524-25
CAGTCAC19650.010.81925726-27
AGCACAC35500.09.85376510-11
CTGAACT35850.09.42257418-19
TGAACTC36350.09.42244420-21
ACTCCAG41200.08.71419222-23
Produced by FastQC (version 0.11.5)