FastQCFastQC Report
Wed 31 Oct 2018
H5FFNAFXY_n01_VS9_181029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_VS9_181029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9699765
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG19003.912646E-96.2508461
CACATGT83500.06.114898794-95
AGTCACA103550.05.825334594-95
GTCACAT98600.05.611982394-95
TCACATG88050.05.55616594-95
CAGTCAC106450.05.35430294-95
CCAGTCA115400.05.288890494-95
TCCAGTC116300.05.28880294-95
GTCTGAA185150.05.066530794-95
CGTCTGA178550.05.02769894-95
TCTGAAC185500.05.018563794-95
Produced by FastQC (version 0.11.5)