FastQCFastQC Report
Wed 31 Oct 2018
H5FFNAFXY_n01_VS8_181029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_VS8_181029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8441689
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT106040.12561467260876347TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA70200.053.6645771
GAGCACA73200.053.6590779
TCGGAAG81550.048.048663
AGAGCAC82600.047.6100968
ATCGGAA90550.043.4830472
CGGAAGA91150.043.196564
GCACACG59000.033.2868110-11
GAAGAGC123250.032.4856836
ACACGTC62500.031.61273412-13
AGTTCCG63250.031.01738234-35
ACGTCTG63450.030.98970614-15
CACGTCT63950.030.97021714-15
CACACGT63650.030.9669512-13
CCGTATC61750.029.77135838-39
GGAAGAG136200.029.1876875
GAACTCC68300.028.61645720-21
CGTCTGA69100.028.45581416-17
GTTCCGT67900.028.43848234-35
CAGTCAC68600.028.32165726-27
AAGAGCA143050.028.0554717
Produced by FastQC (version 0.11.5)