FastQCFastQC Report
Wed 31 Oct 2018
H5FFNAFXY_n01_VS6_181029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_VS6_181029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4712229
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA33800.035.1291439
GATCGGA37000.031.2049831
AGAGCAC39150.030.6925498
TCGGAAG42350.028.4858463
CGGAAGA46800.025.6757724
ATCGGAA46850.025.5475352
ACACGTC25350.023.88781512-13
GCACACG25200.023.65306310-11
ACGTCTG26100.023.11040114-15
CACGTCT27950.021.66569314-15
CACACGT27700.021.5183112-13
CAGTCAC28150.021.34655426-27
GAACTCC29450.020.56250620-21
CGTCTGA29800.019.9222316-17
TCCAGTC30250.019.7836124-25
GAAGAGC61350.019.7412416
GTCACCT31850.019.31631928-29
CCAGTCA31650.019.06126-27
AGCACAC31700.018.95289210-11
AGTCACC32300.018.7530428-29
Produced by FastQC (version 0.11.5)