FastQCFastQC Report
Wed 31 Oct 2018
H5FFNAFXY_n01_VS4_181029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_VS4_181029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7989961
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA38850.026.5343881
GAGCACA46150.023.5676969
GTCACGC23900.022.16221828-29
AGAGCAC50500.021.4435588
TCACGCC27100.019.54533830-31
TCGGAAG55650.019.4592363
AGTCACG28850.018.6890128-29
GCACACG30400.017.96708510-11
CGGAAGA64450.016.949664
ATCGGAA64300.016.5463142
ACACGTC34350.016.1775412-13
CACACGT35450.015.47458812-13
TATCTCG32400.015.24836338-39
ACGTCTG35900.015.21446714-15
CACGCCA35100.014.95521930-31
CACGTCT37250.014.66307114-15
CGTCTGA38250.014.40389316-17
CAGTCAC41850.013.50713626-27
TATGCCG32050.013.488317546-47
GAACTCC41100.013.34776320-21
Produced by FastQC (version 0.11.5)