FastQCFastQC Report
Wed 31 Oct 2018
H5FFNAFXY_n01_VS2_181029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_VS2_181029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8296717
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC139080.16763257081084001TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA58650.031.4204779
GATCGGA57400.031.0374681
AGAGCAC67850.027.1600748
TCGGAAG71650.026.3165283
ATCGGAA79550.023.2852752
CGGAAGA84000.022.108124
GCACACG41900.021.8772310-11
ACACGTC44900.020.83862112-13
ACTGACC44200.020.2071532-33
CACACGT46700.019.83201612-13
ACGTCTG46750.019.65841314-15
GTATGCC43500.019.33061244-45
GCCGTCT36600.019.14512448-49
CGTCTGA48350.019.00787516-17
CGTATGC44750.019.00297744-45
TGCCGTC40700.018.9673348-49
TATGCCG44000.018.89500446-47
CACGTCT49100.018.76589614-15
GAACTCC49350.018.33426320-21
GAAGAGC104200.018.2780976
Produced by FastQC (version 0.11.5)