FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_VS16_181029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_VS16_181029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8536617
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA45250.016.0590299
GATCGGA44650.015.8530571
AGAGCAC53200.013.659238
TCGGAAG58100.012.7525633
ATCGGAA65150.011.3726512
GCACACG33400.010.949409510-11
ACGTCTG36700.010.28839214-15
CGGAAGA76000.010.1864534
ACACGTC36200.09.90569212-13
CACACGT37950.09.82436212-13
CACGTCT37950.09.574059514-15
AGCACAC40050.09.19063810-11
CAGTCAC41350.09.0755826-27
TATCTCG28000.08.99172840-41
TCCAGTC42500.08.94096524-25
AGTCACA43900.08.92776528-29
GAACTCC43400.08.91940720-21
GTATGCC32050.08.670958546-47
CCAGTCA43850.08.50399226-27
GTCACAT45050.08.22532628-29
Produced by FastQC (version 0.11.5)