FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_VS14_181029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_VS14_181029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7288533
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT74130.10170770990540895TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA38550.022.9162719
AGAGCAC45100.019.2721398
GATCGGA41300.019.2102071
CACGAGT23000.017.86611430-31
TCGGAAG53050.015.9365013
ACGAGTG27300.015.31342132-33
GCACACG29050.014.55121510-11
ACACGTC30750.014.44181812-13
AGTCACG29650.014.41981228-29
ATCGGAA60950.013.8709952
TCACGAG30700.013.77188630-31
GTCACGA31150.013.72543828-29
TATCTCG30200.013.52715540-41
CACACGT33200.013.37608112-13
ACGTCTG33250.012.92743414-15
CACGTCT34100.012.88376314-15
CGGAAGA68950.012.6748234
GCCGTCT28350.012.65040350-51
GTATGCC33800.012.29737246-47
CGAGTGG36750.011.89276132-33
Produced by FastQC (version 0.11.5)