FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_VS12_181029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_VS12_181029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6478206
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT446840.6897588622529138TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA98700.057.4565669
AGAGCAC107550.052.905278
GATCGGA110800.049.739131
TCGGAAG118350.048.037653
CGGAAGA122050.046.5424544
ATCGGAA127000.044.8031962
GAAGAGC140950.040.2341776
AGTCACG71000.039.9121628-29
CACGTGA73650.038.57283430-31
GTCACGT73150.038.47929828-29
TGCCGTC73100.037.8201750-51
CGATCTC66350.037.6228240-41
TCACGTG75900.037.42936730-31
GGAAGAG153350.037.2905355
GCCGTCT73050.036.7405950-51
AAGAGCA157500.036.307687
CGTATGC77500.036.25707246-47
GATCTCG69400.035.79825240-41
ATGCCGT79600.034.73264748-49
GTATGCC80350.034.70498746-47
Produced by FastQC (version 0.11.5)