FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_VS10_181029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_VS10_181029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9263053
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT212620.22953555377476517TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG38350.036.9731134-35
GTCCCGA38100.034.59808336-37
ACCCGTC44850.031.45887832-33
CCCGTCC47050.030.34129332-33
TCACCCG48450.029.07172230-31
CACCCGT50550.028.05194730-31
CCGTCCC53500.026.50315334-35
CCCGATC43700.025.70828638-39
TGCCGTC52800.025.46102750-51
TCCCGAT47450.025.42796336-37
GTATGCC55050.025.41410646-47
CCGATCT45100.024.85758238-39
GAGCACA119550.024.552429
CGATCTC46000.024.2685640-41
GCCGTCT52900.024.02102550-51
CGTATGC57600.024.00033846-47
ATGCCGT58900.023.75252748-49
GATCTCG48400.023.75220740-41
GTCACCC64050.022.69562528-29
CAGTCAC67250.021.33191726-27
Produced by FastQC (version 0.11.5)