FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_AH9847input-3h-1018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_AH9847input-3h-1018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6500990
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC24100.021.97925632-33
ACTTAGG25700.020.1487732-33
GAGCACA52600.019.7746289
AGAGCAC58450.018.1205048
GATCGGA62000.016.2449251
TTAGGCA33450.015.6209834-35
TAGGCAT34300.015.02613734-35
TCGGAAG70300.014.9986073
CAGTCAC35150.014.66294326-27
CACTTAG36400.014.29056930-31
TCCAGTC36800.013.9396224-25
GCACACG38100.013.7125310-11
CACGTCT39050.013.56137714-15
ATCGGAA79400.013.5191032
CCAGTCA37950.013.45592126-27
ACGTCTG39050.013.31812414-15
CGGAAGA82550.013.1756384
ACACGTC39350.013.03553812-13
CGTCTGA40050.012.98558616-17
AGTCACT40900.012.89248128-29
Produced by FastQC (version 0.11.5)