FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_AH9847input-0h-1018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_AH9847input-0h-1018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8190905
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC151200.18459498675665265TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA62150.031.4852189
AGAGCAC68750.028.2553848
GATCGGA69200.027.2531831
TCGGAAG78950.024.6053623
GCCGTCT40400.022.1061648-49
ATCGGAA90500.021.8324932
TGCCGTC42450.021.59814548-49
CGATCTC37100.021.38274638-39
CACGATC37850.021.20985636-37
CATCACG45300.021.1846132-33
CGGAAGA93950.020.8791054
GATCTCG37900.020.6807238-39
GTATGCC46250.020.5941344-45
GCACACG48550.020.15248510-11
CAGTCAC49700.019.88034226-27
ACGTCTG48750.019.82624214-15
GAACTCC50200.019.53782820-21
CGTATGC48800.019.51800244-45
ACACGTC50250.019.32893412-13
CACGTCT50500.019.23324614-15
Produced by FastQC (version 0.11.5)