FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_AH9847Myc-3h-1018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_AH9847Myc-3h-1018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8065441
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA81150.057.7672169
AGAGCAC83050.056.903148
GATCGGA80800.056.0919081
TCGGAAG94200.049.966423
ATCGGAA104150.045.1931462
CGGAAGA110650.042.8385354
GAAGAGC119150.039.941946
GGAAGAG133350.035.8311275
GCACACG66800.035.08839410-11
ACGTCTG67650.034.5773114-15
AAGAGCA137750.034.548697
ACACGTC68350.034.53589212-13
CACGTCT67850.034.44038814-15
CACACGT70800.033.20662712-13
CGTCTGA71550.032.85854716-17
CACTAGC72200.032.50440230-31
CAGTCAC74550.031.5411626-27
TATCGGG37950.031.29259738-39
TCCAGTC74550.031.28317824-25
GAACTCC75650.031.23544920-21
Produced by FastQC (version 0.11.5)