FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_AH9847Myc-0h-1018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_AH9847Myc-0h-1018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8597896
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC447150.5200690959741779TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA85300.063.1397939
AGAGCAC92900.058.12788
GATCGGA90200.057.779411
TCGGAAG100550.053.990013
ATCGGAA112950.048.147482
CGGAAGA114450.047.1839034
GAAGAGC131950.041.3940286
AAGAGCA144500.037.863767
CGATCAG72200.037.3110832-33
AGTCACG72350.037.1992728-29
GCACACG73300.036.8354210-11
GGAAGAG148500.036.7167855
GTCACGA73650.036.38139728-29
ACACGTC76150.035.55036512-13
CACGATC75800.035.3809130-31
ACGTCTG76850.035.0720514-15
GATCTCG69850.034.88748638-39
CACACGT77550.034.81671512-13
TGCCGTC75400.034.80875448-49
GTATGCC77100.034.5655644-45
Produced by FastQC (version 0.11.5)