FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_AH7797input-3h-1018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_AH7797input-3h-1018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10175464
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC49100.017.90001328-29
GAGCACA98550.017.880089
ATCGGGG26500.017.38739640-41
AGAGCAC105400.016.5377948
AATCGGG35650.015.25630338-39
CAGTCAC60150.014.88684126-27
GAATCGG39050.014.29289438-39
CGGGGGT10600.014.1160942-43
TCGGGGG13300.013.75047740-41
TCCAGTC66300.013.68347724-25
CCAGTCA64850.013.51491126-27
AGTCACA64250.013.49437728-29
CGGGGTG16900.013.49172550-51
ACGTCTG66700.013.137814-15
GAACTCC68850.012.83142920-21
TCACACT69800.012.65961830-31
CGTCTGA70350.012.52367916-17
TCGGAAG137450.012.5088093
ACACGTC73450.012.25376412-13
ACACTTG72700.012.22015532-33
Produced by FastQC (version 0.11.5)