FastQCFastQC Report
Tue 30 Oct 2018
H5FFNAFXY_n01_AH7797Myc-3h-1018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFNAFXY_n01_AH7797Myc-3h-1018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7041737
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA28700.030.6252421
GAGCACA30800.028.8360399
AGAGCAC35600.024.8146238
GTCACGG18200.023.49145528-29
CACGGCT18550.023.43260830-31
TCGGAAG39700.023.0895043
TCACGGC20000.022.09003330-31
GCACACG19900.021.7186610-11
AGTCACG20850.020.84749228-29
CGGCTAC22350.019.7682832-33
ATCGGAA48800.019.270922
ACACGTC23700.018.7373412-13
ACGGCTA25500.017.60577232-33
CACACGT25000.017.38304312-13
ACGTCTG25450.017.07568214-15
CACGTCT26450.016.60966114-15
CGGAAGA55750.016.4422114
CGTCTGA27800.016.05934316-17
GCCGTCT22150.015.22791448-49
AGCACAC29400.014.86227310-11
Produced by FastQC (version 0.11.5)