FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6221968
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373760.6007102575905244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCA28400.013.57384626-27
CCTTAGA9300.012.714727
ACTCTAG5603.8180588E-912.6734865
CTCCAGT37200.012.588078524-25
CAGTCAC25650.012.26303426-27
CTAGACT8250.012.040078
GAGGTCA8300.011.9716772
CTTAGAG9500.011.9495438
CACCTTA10700.011.9391345
CCGGGAC4103.826066E-511.5454671
CGGGAAT8205.456968E-1211.5454671
AGAGCAC76850.011.4480828
TCTAGAC8851.8189894E-1211.22343357
ATGCCGT15700.011.1506034
ATACGGC6654.8483344E-810.6724095
ACCTTAG12000.010.6457286
TGATACG6252.3668508E-710.5989253
AACTCCA54750.010.36805922-23
CCGGAAT9852.0008883E-1110.0920281
CGGGCAT7051.13277565E-710.0715781
Produced by FastQC (version 0.11.5)