FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-WV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-WV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8450329
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA60800.013.3591788
GTACTAA62750.012.9440329
CCTTTCG65400.012.9280193
TCCTTTC72350.012.0802732
CTCGTCT74850.012.0631941
TTCGTAC69500.012.028676
CTTTCGT71900.011.4950294
GTCCTTT73750.011.469871
CGTCTTT78150.011.3050813
CGCAAAT60100.011.1492371
TCGTCTT85850.011.065912
TTTCGTA83350.010.65677455
CGGAAAT48700.010.53891
TACACCC75800.010.4022825
CTCAAAT59900.010.2344241
GACCTAG8601.9135769E-99.94177155
GCTACAC78500.09.8629693
ACCCTAT41850.09.8178384-85
CGGGTCT23800.09.7840711
CGGCTAC85100.09.4933581
Produced by FastQC (version 0.11.5)