FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-WA3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-WA3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8612363
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1392461.6168152689337407TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA167080.19400018322497553TruSeq Adapter, Index 13 (97% over 40bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT134890.15662368156103035No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA100940.1172036060254311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG238050.034.78935248-49
CGTATGC246950.034.0744246-47
CTCGTAT252750.032.7569844-45
CCGTCTT211700.032.52276252-53
CTTGAAA196650.032.51162362-63
TGCCGTC235100.032.1344750-51
AAAGGGG120350.031.83058272-73
AGGGGGG142900.031.42800974-75
TGCTTGA186650.030.88138860-61
GTATGCC246700.030.12331646-47
TCGTATG251600.029.26298944-45
GCCGTCT204000.029.256550-51
CAATCTC303750.029.21213340-41
ATGCCGT239250.029.14517848-49
ATCTCGT301400.028.85669142-43
GTCTTCT204150.028.60646654-55
GCTTGAA193450.028.43314460-61
AACAATC334450.028.1639938-39
TGAAAAA311650.027.89909764-65
TCTCGTA267850.027.74503942-43
Produced by FastQC (version 0.11.5)