FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-WA2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-WA2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8942317
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC202360.22629481822216768TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC170500.19066646820952557TruSeq Adapter, Index 12 (98% over 50bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT122020.13645233109047689No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG76000.026.71826470-71
CGTATGC94500.025.91155644-45
TATGCCG90900.025.89264546-47
ACCTTAG62300.025.2362986
AGGGGGG90400.025.16823472-73
AAGGGGG77450.025.08346772-73
CACCTTA64700.024.1533475
GTATGCC98450.022.48364844-45
CTTGTAA283100.022.14854834-35
CCTTAGA70550.022.01597
TGTAATG160800.021.97850236-37
ACTCTAG35000.021.713895
TCGTATG99750.021.59556842-43
TAATGTC98850.021.28786838-39
CTTAGAG75650.020.594478
CTCTAGT36500.020.4311356
TTAGAGT78350.019.9453939
ACCTTGT316000.019.8949132-33
GTCGTAT70100.019.88796442-43
TAATCTC95350.019.72784238-39
Produced by FastQC (version 0.11.5)