FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-WA1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-WA1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7388487
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC910721.2326204268884822TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG139300.18853657047782585TruSeq Adapter, Index 11 (100% over 49bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT127180.17213267073488794No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA92880.1257090930795439No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG175950.033.6246746-47
CGTATGC184100.032.87147544-45
CTCGTAT183150.031.99212842-43
CCGTCTT160500.030.94188150-51
CTTGAAA152150.030.56325560-61
TGCCGTC176000.030.5245448-49
TGCTTGA140850.029.96328558-59
AAAGGGG78350.029.79665670-71
AGGGGGG96250.029.36291972-73
GTATGCC183600.028.8085344-45
ACATCTC215100.028.18985238-39
TCGTATG185400.028.05532642-43
GCCGTCT150750.027.93319548-49
ACTCTAG34550.027.7706685
ATCTCGT214400.027.73916640-41
ATGCCGT179750.027.60216346-47
CTACATC234100.027.2109836-37
GCTTGAA147150.026.90507758-59
TCTCGTA191350.026.53769140-41
TCTGCTT156900.025.88412756-57
Produced by FastQC (version 0.11.5)