FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-OV3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-OV3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12391859
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAATATTAACTGTTTCATTTAAAATTTTTATAGAATTTTATTTTTTAG125810.10152633273183627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCT106850.012.0088341
CGTACTA88950.011.9614558
ACCCTAT62300.011.3603784-85
GTACTAA93400.011.3407029
CCTTTCG101150.010.8945953
CGTCTTT114650.010.7717993
TTCGTAC104300.010.6566456
CGCAAAT90250.010.53161
GACCCTA61700.010.31606184-85
CCGGGAT47350.010.237451
CTCAAAT90650.010.2230011
CCCTATA61500.010.19493186-87
ACTCTAG17750.010.1689055
CGGCTAC132100.010.1451361
GTCCTTT107900.09.9980671
TACACCC118700.09.9640895
GCCGGTT38400.09.9008011
CGGGAAT68250.09.8877461
CGGGGAT41350.09.884041
CTTTCGT112750.09.7737324
Produced by FastQC (version 0.11.5)