FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-OV2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-OV2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9339395
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT145250.1555239927211559No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT123420.13214988765332228TruSeq Adapter, Index 19 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA100130.10721251215951354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG36200.029.7855115
ACCTTAG60350.027.4685636
CTCTAGT41050.027.0763726
CCTTAGA69700.024.328557
CACCTTA69150.024.1103065
CTAGTTG48650.022.5532918
CTTAGAG75300.022.0777028
TCTAGTT54900.021.7161187
TTAGAGT81100.020.6159139
TACTCTA60500.018.136124
GTACTCT61650.017.7978153
TTCCACC100950.017.0803742
TTTCCAC100100.016.6642741
TATGCCG57900.015.54665848-49
CGTATGC58650.014.90259846-47
AGGGGGG26550.014.5805574-75
CCACCTT120550.014.1453934
TAGTTGT84850.013.4910589
TGTACTC81400.013.3631292
TCCACCT126700.013.3463083
Produced by FastQC (version 0.11.5)