FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-OV1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-OV1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8068090
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT180440.22364648882201363No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT140850.17457663461860243TruSeq Adapter, Index 18 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA127740.15832743561363347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG47950.031.2037755
CTCTAGT48850.029.656546
ACCTTAG79700.027.116796
CACCTTA79850.026.9468775
TCTAGTT55650.026.6298797
CCTTAGA81650.026.2943257
CTAGTTG55950.025.7230428
CTTAGAG85800.025.0225148
TTAGAGT91500.023.3599139
TACTCTA68700.020.8110944
TTCCACC106550.020.5511132
TTTCCAC103950.020.390211
GTACTCT69250.020.3714453
TTTTTCC89000.017.674591
CCACCTT123200.017.5422734
TATGCCG51000.017.46343648-49
TCCACCT126500.017.4225863
TGTACTC82950.017.3505842
TAGTTGT83550.017.2825139
CGTATGC51950.016.96137446-47
Produced by FastQC (version 0.11.5)