FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-OA3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-OA3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7185216
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG24100.020.3004825
ACCTTAG33050.020.1206846
CTCTAGT25800.018.9628526
CTAGTTG28350.017.9271348
CACCTTA38450.017.2948935
TCTAGTT34750.016.4023697
CCTTAGA43000.015.2437127
CTTAGAG43800.014.5315138
TACTCTA33400.014.22131254
TTAGAGT48000.013.7547829
GTACTCT36700.013.0719833
CGCAAAT50400.012.6355211
AGAGTAC47950.011.68889610-11
AAGGGGG19450.011.47787274-75
CGTACTA37350.011.3182558
AGGGGGG20600.011.2982774-75
CTCAAAT56850.011.2855371
CGGGAAT39050.010.9531851
GTACTAA39350.010.8637049
TTCCACC63000.010.6310652
Produced by FastQC (version 0.11.5)