FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-OA2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-OA2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8930353
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAG44150.020.5489986
ACTCTAG29700.020.3111845
AAGGGGG35400.018.98633874-75
CACCTTA51300.018.7034635
CTCTAGT34050.017.7163646
CTTAGAG53900.017.272318
CCTTAGA55850.016.4991557
TTAGAGT59000.015.6182679
CACTACG23950.015.4697032
CTAGTTG38550.015.03206358
ATCGGGG72150.014.81344642-43
TCTAGTT44500.014.729987
AGGGGGG39150.014.19545776-77
ACTACGC28550.013.9753013
GCGGGGT38450.013.21859252-53
CGGGGGT31850.013.12425344-45
GTCAGAA163050.013.02253136-37
CGGGGTT34250.012.89804144-45
AATCGGG79750.012.59775640-41
CAGAATC162500.012.51127338-39
Produced by FastQC (version 0.11.5)