FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Fly-OA1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Fly-OA1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9029004
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT194050.2149185004237455No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA138670.15358283150611077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG44050.033.8587575
CTCTAGT48000.031.1714216
ACCTTAG78800.026.2210676
CTAGTTG57550.026.0805688
TCTAGTT62600.025.4942727
CACCTTA88900.023.6160815
CCTTAGA90400.022.7508267
CTTAGAG94500.021.8641578
TTAGAGT99350.020.8924269
TACTCTA74000.019.8983614
TTTCCAC105300.019.5443441
GTACTCT75150.019.5306553
TAGTTGT85050.018.7087929
TTCCACC116550.018.2583982
TTTTTCC94050.017.1823161
TGTACTC92350.016.3049432
CCACCTT140050.015.0926054
CTACCCT27650.014.6016347
TCCACCT150900.014.0703733
AGAGTAC111800.013.89252610-11
Produced by FastQC (version 0.11.5)