FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Ant-Tm8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Ant-Tm8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7540148
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC79070.10486531564101924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA35700.021.2883344
GAGGTCA37350.019.585492
TAATCCC46000.017.2440769
AGGTCAT41500.017.1685283
TGGGGGG26450.016.351451
GTCATAA53950.014.3511285
TCTAGAC52050.014.3271647
CTAGACT51450.014.0326428
CGAGGTC54100.013.6167971
CGGGAAT39400.013.5102181
ATTCTAG65350.012.4291215
ATAATCC66800.012.2301918
TTCTAGA67450.011.5491976
TAGACTT62450.011.4088039
CATTCTA75350.011.0317574
GGGAATC28900.010.3549262
CTCGGGT22200.010.2761121
GGGGAAT17700.09.3979981
CCCTAAG44600.09.37217954-55
CCCGTAT16300.09.3304571
Produced by FastQC (version 0.11.5)