FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Ant-Tm7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Ant-Tm7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9358015
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC96610.10323770585962942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA41750.020.1374034
GAGGTCA48650.015.9147852
CTAGACT67400.015.5745128
AGGTCAT50200.015.5176833
TCTAGAC68800.015.4647067
TAATCCC58950.014.2617079
TGGGGGG34050.013.9575331
GTCATAA64600.013.2350855
TAGACTT82400.012.9699239
ATTCTAG89300.012.7125655
TTCTAGA91850.012.2562026
CATTCTA96900.012.156674
CGAGGTC66900.011.5083931
CGGGAAT49400.011.1598111
GGGAATC35100.010.6909422
CCCATAA64200.010.4319669
CGTCCCA64900.010.3195596
ATAATCC86950.010.2699988
CCCGGAT25900.09.90877151
CCGGGAT30950.09.8275471
Produced by FastQC (version 0.11.5)