FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Ant-Tm6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Ant-Tm6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9295569
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC126240.1358066407769121No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA100970.10862164543128021No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT100830.10847103603878364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA48000.022.759944
GAGGTCA52300.017.8008692
TAATCCC60750.017.7484079
TGGGGGG29900.017.4845181
CTAGACT83100.016.861688
AGGTCAT56450.016.4079823
TCTAGAC85850.016.2662287
GTCATAA73250.014.9792075
TAGACTT96800.014.57340059
GGGAATC32750.014.5035912
CGGGAAT42700.014.3580021
CGAGGTC63850.014.2913261
GGGGAAT19050.013.2224861
ATTCTAG108650.013.2026685
TTCTAGA108850.013.1347736
ATAATCC89150.012.787038
CATTCTA120800.012.5038774
GACCCTA32500.011.1073397
CCCAATT69000.010.8827891
AGTGACC23300.010.8044956
Produced by FastQC (version 0.11.5)