FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Ant-Tm5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Ant-Tm5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9598684
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC112360.11705771332820207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA46300.020.5181454
GAGGTCA50850.017.0009842
AGGTCAT52350.016.3322093
TAATCCC62750.014.9120419
CTAGACT73950.014.7089588
TCTAGAC76800.014.5342047
GTCATAA68450.014.0867795
TGGGGGG33100.012.778761
ATTCTAG92400.012.6973795
TAGACTT87400.012.5540889
CATTCTA100200.012.4674364
TTCTAGA96650.011.9915986
CGAGGTC73500.011.7035591
ATAATCC89200.010.6500038
CCGGGAT35350.010.62097551
CCGGAAT35500.010.1744741
GACCCTA28700.010.0957155
CGGGAAT47500.010.0053681
GGGGAAT21800.09.810311
CGGGCAT25250.09.7874311
Produced by FastQC (version 0.11.5)