FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Ant-Tm4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Ant-Tm4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12274307
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC171120.13941316605491455No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA136760.11141973229119984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA64450.021.5201974
TGGGGGG40100.017.3032781
TCTAGAC111350.017.0200927
CTAGACT110400.016.7793378
TAATCCC80900.016.6156439
GAGGTCA72550.016.4989592
GTCATAA87300.016.1595235
AGGTCAT74150.016.0145663
CGGGAAT65100.014.6005391
ATTCTAG134900.014.5420085
TAGACTT128950.014.4760639
TTCTAGA138850.014.2651546
CATTCTA151300.013.8133824
CGAGGTC92750.013.2198251
AGTGACC35600.012.9421996
GGGAATC49550.012.9414662
ATAATCC116200.012.1403048
TGACCGC39200.011.3899298
TCATTCT199800.010.4840573
CCCAATT92150.010.3662241
Produced by FastQC (version 0.11.5)